Comparing this study to that of Burrows and Hoch-Ligetti, the dose of 2 mg per week per rat is lower, on a body-weight basis, than the sooner study’s dose of 1 mg per week in mice, however the larger frequency came just a little nearer to the continuous treatment that Lipschutz said was mandatory. Despite the unexplained normal downside with prematurely dying rats, the authors found that “The relative incidence charges indicated that pretreatment with progesterone inhibited tumorigenesis, except in the group (5) in which progesterone remedy was continued during the experiment.” Without progesterone, it is sort of certain that the additional corn oil injected would have increased tumorigenesis in all experimental teams. One of the teams (group 5) obtained, as well as, more than 6 ml of corn oil in the injections. That quantity of progesterone (3% weight/quantity) will dissolve in scorching corn oil, however as the oil cools, the progesterone crystallizes and precipitates. The animals bought 2 ml of corn oil in the stomach feeding with the carcinogen.
Invasive squamous carcinomas had been produced by the carcinogen 20-methylcholanthrene, and these have been extra numerous within the progesterone handled mice. This research may need had the formal appearance of a scientific experiment, but the unfamiliarity of the males with the material they have been utilizing, their use of mice carrying the mammary tumor virus, and, extra importantly, the extremely complex reactions produced when extraneous materials are injected into the tissues, make this a useless experiment. Abundant and fluid mucus has a cleaning motion, eliminating bacteria and different material. Progesterone makes the mucus more viscous and less hydrophilic, and when it dominates the reproductive physiology, it successfully creates a plug within the cervix that prevents the entry of sperms. The choice of the cervix and vagina suggests that the authors had been “engineering” the experimental final result, as a result of the impact of progesterone on cervical mucus may be very well-known. Estrogen increases the manufacturing of mucus in the cervix and vagina, and increases its water content and mobility. They commented (page 74) that their outcomes had been “mammary tumor virus dependent,” and that this might account for the production of “hyperplastic alveolar nodules as opposed to” tumors of attainable ductal origin, that had been seen in other research when the carcinogen DMBA was used.
Tissue irritation from foreign bodies and from vegetable oil, even in comparatively small quantities, can produce severe systemic reactions, because of the reactive manufacturing of nitric oxide, prostanoids, and an awesome number of pro-inflammatory and tumor-selling cytokines. The value of Richard Edgren’s statement about the need to check carcinogens orally, rather than by injection, is changing into clearer on a regular basis, because the function of irritation in cancer development is being higher understood. Anyone working with mammary tumor virus-bearing mice within the 1970s ought to have been conscious of the consequences of sex hormones on the expression of virus and growth of cancer in the contaminated mice, as studied by Strong, Figge, and others for about 40 years. The 1977 examine (at the University of California, Berkeley) was explicitly motivated by Jones’ and Bern’s concern with the risks of the medical observe of treating pregnant girls with DES and a artificial progestin, they usually used mammary tumor virus-bearing mice, they usually did not proceed the examine to observe the incidence of precise cancers. This later publication seems to make clear the which means of nodules or tumors in the younger animals: “Although mammary tumors have been observed neither in control nor in progesterone-treated intact mice, most of the latter group possessed hyperplastic alveolar-like mammary nodules and other dysplasias.” Neither of those research refers to the carcinogenicity of progesterone.
Without that car control group, the experiment can simply as nicely be described as a test of corn oil, fairly than of progesterone. On this experiment, the carcinogen was administered to rats in one dose by stomach tube, dissolved in corn oil. The experiment lasted only 135 days, and in the group that acquired solely the carcinogen, the mortality was only 5%, and that death occurred shortly after the carcinogen was administered. Benzyl alcohol by itself is sort of toxic, and was chargeable for the demise or brain injury of 1000’s of babies in hospitals, even in the small quantities that remained as residue in tubing after they had been rinsed with “bacteriostatic water,” which contains 0.9% benzyl alcohol, and which continues to be used because the automobile for a lot of injections, comparable to penicillin and vitamin B12. Even with a free membership, you can nonetheless use the web site and search for sugar babies or sugar daddies online.